Figure 6. DR Alters the Polarization of Intestinal Stem Cells (ISCs) toward Different Lineages.

(A) Intestinal stem and progenitor cells were isolated from the whole SI of Lgr5-eGFP-CreER mice and analyzed by quantitative proteomics.

(B) Principal-component analysis of Lgr5^high ISCs and Lgr5^low progenitor cells based on protein intensities measured by DIA mass spectrometry (n = 12).

(C and D) Abundance changes for markers of stem cells and differentiated cells in Lgr5^high ISCs from old animals that underwent DR (C) or DR+RF (D) (n = 4).

(E and F) Abundance of Muc2 (E) and Lyz1 (F) in Lgr5^high ISCs of AL, DR, and DR+RF animals shown as normalized protein intensity (n = 4, mean of AL = 1).

See also Figure S6 and Table S5.