Fig. 4. Ubiquitination study of HA-p19INK4d analogues. Immunoblot of each synthetic analogue of HA-p19INK4d with addition of S-phase U2OS lysate obtained before incubation in 37 °C (0 h) and after 1 h. The control contained assay compounds with cell lysate. Actin is shown below as a loading control. *Unidentified bands which can correspond to impurity and poly-ubiquitinated p19INK4d.
