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. 2020 Aug 10;16(8):e1008646. doi: 10.1371/journal.ppat.1008646

Fig 5. IPPK-KO cells expressing MINPP1 have substantial loss in infectious particle production due to a block in viral release.

Fig 5

(A) Experimental timeline. (B) Bar chart of percent infectious particle release normalized to virus from HEK293FT cells expressing the empty vector. Student’s t-test was used for pair-wise comparison (n = 5, *** p < 0.001, error bars = mean ± SD). (C) Representative western blot of two experiments from panel B. The rows are full-length HIV-Gag (top), GAPDH loading control (middle), and virus released into media (bottom). A longer exposure was also taken for the blot of released virus. (D) Relative quantification of uncleaved pr55 Gag normalized to GAPDH levels in cell lysate. (E) Relative quantification of p24 Gag in virus released into media.