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. 2020 Aug 10;16(8):e1008646. doi: 10.1371/journal.ppat.1008646

Table 1. Primers used for cloning.

Primer Sequence
IPPK guide RNA forward caccgAACAGCGCTGCGTCGTGCTG
IPPK guide RNA reverse aaacCAGCACGACGCAGCGCTGTTc
IPMK guide RNA forward caccgTCACCTCCCACTGCACCAAA
IPMK guide RNA reverse aaacTTTGGTGCAGTGGGAGGTGAc
ITPK1 guide RNA forward caccgGGCCCTGCTCCTCGATCGGC
ITPK1 guide RNA reverse aaacGCCGATCGAGGAGCAGGGCCc
IPPK cDNA forward cctcgtacgcttaatATGGAAGAGGGGAAGATGGACG
IPPK cDNA reverse gcggaattccggatcTTAGACCTTGTGGAGAACTAATGTGC
MINPP1 cDNA forward cctcgtacgcttaattaaATGCTACGCGCGCCCGGC
MINPP1 cDNA reverse gcggaattccggatccTCATAGTTCATCAGATGTACTG
IPPK flanking forward GAAATGTGTGCCACTGTGTTTA
IPPK flanking reverse ATGATGGACACACCACTTTCT
IPMK flanking forward AGGCTAGAATTAGATAACCAAGAAGAG
IPMK flanking reverse GAGGAAGTCATGCAGAGACAATA
ITPK1 flanking forward CCTGGCCTGTTGACACTATT
ITPK1 flanking reverse GAGCCATTTCTCCAGACTATACC