Fig 5. Three immunizations of mice with 4X-SA-GP induces robust antibody responses.

(A and B) Serum was collected from each group of vaccinated mice 2 weeks after the third and final immunization with PBS, Empty-GP, or 4X-SA-GP (n = 10 mice per group). The serum was diluted 10X 3 times starting at 1:1000 and was then tested for antibodies specific for each of the 4 S. aureus proteins encapsulated in 4X-SA-GP by ELISA. The subclasses IgG1 (A) and IgG2c (B) were analyzed for specificity towards rClfA, rIsdA, rMntC, and rSdrE. The read-out of the assay is the optical density (OD) at 450 nm for each serum sample. Each data point represents an individual mouse. Data are representative of three experiments and were analyzed using ANOVA at each dilution. ****p<0.0001.