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. 2020 Aug 12;16(8):e1008835. doi: 10.1371/journal.ppat.1008835

Fig 3. The EAR motif is necessary for RxL21 virulence function in planta.

Fig 3

Transgenic lines expressing RxL21 under control of a 35S promoter and N-terminal HA tag (HA:21#1/2 and HA:21ΔEAR#1/2) were compared to Col-4 WT and HA:GFP (Col-4 background) controls during infection with (A) Hpa isolate Noks1 (sporangiophore counts per seedling at 4 dpi). Letters indicate significance using a Kruskal Wallis test, p < 0.05, n = 45 (B) B. cinerea. Lesion area was measured at 72 hpi (letters indicate significance determined by a one way ANOVA, p < 0.05, n = 24). Error bars display 95% CE. (C) Estradiol inducible lines expressing RxL21 and RxL21ΔEAR fused to an N-terminal Myc tag (Est:21#2/9 and Est:21ΔEAR#1/2) were infected with Hpa 18 hours after induction with 30 μM β-estradiol or mock treatment (n = 100 per treatment). (D) The same lines with estradiol or mock treatment were subsequently infected with B. cinerea. Lesion area was measured at 72 hpi (n = 30). Letters in (C) and (D) indicate significant difference between treatments using a 2-way ANOVA and Bonferroni’s multiple comparison test. P < 0.05. Whiskers show data range. Experiments were repeated with similar results.