Table 1. Proteins (n = 35) showing significant difference between the plasma and serum EV samples (Anova with Kruskall-Wallis test followed by Dunn´s test, p-value ≤ 0.05) are shown.
Five keratins listed here were not excluded although they are considered to originate from exogenous sources such as skin or hair.
| Kruskall-Wallis | ||||||
|---|---|---|---|---|---|---|
| Group | Mean | SD | ACD | Citrate | EDTA | |
| p-value | p-value | p-value | ||||
| Alpha-1-antitrypsin | Citrate | 21128.5 | 3673.3 | 0.174 | ||
| EDTA | 57249.3 | 23861.6 | 0.571 | 0.054 | ||
| Serum | 84532.5 | 5897.2 | 0.141 | 0.005* | 0.365 | |
| APOC4-APOC2 readthrough (NMD candidate) | Citrate | 2899.5 | 269.1 | 0.308 | ||
| EDTA | 14746.6 | 3666.4 | 0.308 | 0.042* | ||
| Serum | 23054.3 | 322.9 | 0.042* | 0.002* | 0.308 | |
| Apolipoprotein A-I | Citrate | 327405.2 | 26276.5 | 0.571 | ||
| EDTA | 763780.0 | 136205.2 | 0.174 | 0.054 | ||
| Serum | 964279.7 | 111475.8 | 0.031* | 0.007* | 0.428 | |
| Apolipoprotein A-II | Citrate | 9432.0 | 1918.8 | 0.428 | ||
| EDTA | 32012.0 | 10122.1 | 0.213 | 0.042* | ||
| Serum | 46145.7 | 3288.2 | 0.042* | 0.005* | 0.428 | |
| Apolipoprotein C-III | Citrate | 12313.4 | 2369.5 | 0.734 | ||
| EDTA | 45020.7 | 29899.0 | 0.089 | 0.042* | ||
| Serum | 40119.1 | 2971.9 | 0.042* | 0.017* | 0.734 | |
| C4b-binding protein beta chain | Citrate | 32830.5 | 4683.5 | 0.308 | ||
| EDTA | 145233.1 | 92104.5 | 0.174 | 0.017* | ||
| Serum | 129147.1 | 21501.1 | 0.089 | 0.007* | 0.734 | |
| Clusterin | Citrate | 15921.9 | 2556.5 | 0.174 | ||
| EDTA | 64159.9 | 2776.6 | 0.113 | 0.003* | ||
| Serum | 26015. | 4840.8 | 1.00 | 0.174 | 0.113 | |
| Complement C1s subcomponent | Citrate | 130601.2 | 18724.9 | 0.308 | ||
| EDTA | 77030.6 | 18048.8 | 0.042* | 0.308 | ||
| Serum | 574764.3 | 80460.7 | 0.308 | 0.042* | 0.002* | |
| Complement factor H | Citrate | 37145.7 | 4413.8 | 0.571 | ||
| EDTA | 97696.5 | 24032.5 | 0.213 | 0.07 | ||
| Serum | 161619.3 | 19798.8 | 0.024* | 0.005* | 0.308 | |
| Extracellular matrix protein 1 | Citrate | 1361.0 | 349.4 | 0.910 | ||
| EDTA | 2848.3 | 764.6 | 0.113 | 0.141 | ||
| Serum | 7871.9 | 5854.9 | 0.009* | 0.013* | 0.3008 | |
| Far upstream element-binding protein 2 | Citrate | 40731.4 | 11563.2 | 0.428 | ||
| EDTA | 130437.5 | 10503.6 | 0.428 | 0.113 | ||
| Serum | 272062.8 | 40803.3 | 0.042* | 0.005* | 0.213 | |
| Fibrinogen alpha chain | Citrate | 286470.0 | 80015.7 | 0.497 | ||
| EDTA | 1040565.0 | 140516.8 | 0.213 | 0.054 | ||
| Serum | 178365.8 | 33284.6 | 0.089 | 0.308 | 0.003* | |
| Fibrinogen beta chain | Citrate | 247084.9 | 82918.2 | 0.571 | ||
| EDTA | 796061.9 | 71036.9 | 0.213 | 0.07 | ||
| Serum | 52282.2 | 24865.0 | 0.07 | 0.213 | 0.002* | |
| Fibrinogen gamma chain | Citrate | 214132.6 | 59581.7 | 0.428 | ||
| EDTA | 681749.2 | 115693.1 | 0.428 | 0.113 | ||
| Serum | 112276.9 | 6000.7 | 0.042* | 0.213 | 0.005* | |
| Ficolin-3 | Citrate | 39691.6 | 6389.57 | 0.571 | ||
| EDTA | 3262480.8 | 16914469 | 0.024* | 0.005* | ||
| Serum | 175056.8 | 89930.4 | 0.213 | 0.07 | 0.308 | |
| Haptoglobin-related protein | Citrate | 72310.9 | 6719.7 | 0.005* | ||
| EDTA | 216281.3 | 208441.5 | 0.308 | 0.07 | ||
| Serum | 121909.9 | 24163.3 | 0.174 | 0.141 | 0.734 | |
| Immunoglobulin heavy variable 5–51 | Citrate | 2005.0 | 146.1 | 0.089 | ||
| EDTA | 27441.8 | 12458.0 | 0.174 | 0.002* | ||
| Serum | 3673.2 | 79.9 | 0.734 | 0.174 | 0.089 | |
| Immunoglobulin kappa variable 3–20 | Citrate | 23823.5 | 1779.9 | 0.571 | ||
| EDTA | 79664.0 | 33562.0 | 0.024* | 0.005* | ||
| Serum | 46655.7 | 3347.3 | 0.213 | 0.07 | 0.308 | |
| Immunoglobulin lambda constant 2 | Citrate | 34925.7 | 9817.4 | 0.365 | ||
| EDTA | 90591.5 | 19342.8 | 0.365 | 0.07 | ||
| Serum | 145174.1 | 28041.9 | 0.042* | 0.003* | 0.258 | |
| Immunoglobulin lambda constant 7 | Citrate | 3043.6 | 1419.7 | 0.428 | ||
| EDTA | 1933.9 | 501.0 | 0.213 | 0.651 | ||
| Serum | 438.4 | 349.6 | 0.007* | 0.054 | 0.141 | |
| Immunoglobulin lambda variable 2–14 | Citrate | 11752.6 | 3612.3 | 0.910 | ||
| EDTA | 21819.8 | 4915.8 | 0.054 | 0.07 | ||
| Serum | 23822.8 | 8356.2 | 0.024* | 0.031* | 0.734 | |
| Immunoglobulin lambda-like polypeptide 5 | Citrate | 67358.0 | 15634.6 | 0.428 | ||
| EDTA | 186777.2 | 68310.8 | 0.113 | 0.017* | ||
| Serum | 164633.0 | 4839.5 | 0.089 | 0.013* | 0.91 | |
| Inter-alpha-trypsin inhibitor heavy chain H4 | Citrate | 2136.6 | 130.5 | 0.910 | ||
| EDTA | 4742.3 | 2796.6 | 0.141 | 0.113 | ||
| Serum | 27310.24 | 1537.1 | 0.013* | 0.009* | 0.308 | |
| Keratin type II cuticular Hb6 | Citrate | 4676.76 | 1131.9 | 0.571 | ||
| EDTA | 226331.9 | 72476.3 | 0.005* | 0.024* | ||
| Serum | 9422.9 | 332.7 | 0.07 | 0.231 | 0.308 | |
| Keratin type II cytoskeletal 1b | Citrate | 6686.7 | 1671.8 | 0.571 | ||
| EDTA | 23137.7 | 6736.6 | 0.089 | 0.024* | ||
| Serum | 25899.7 | 3549.7 | 0.07 | 0.017* | 0.91 | |
| Keratin type II cytoskeletal 3 | Citrate | 8968.3 | 1474.3 | 1.00 | ||
| EDTA | 16936.0 | 5221.0 | 0.571 | 0.089 | ||
| Serum | 25805.0 | 3225.4 | 0.089 | 0.005* | 0.258 | |
| Keratin type II cytoskeletal 7 | Citrate | 9786.3 | 5026.1 | 0.428 | ||
| EDTA | 28739.1 | 9806.1 | 0.428 | 0.113 | ||
| Serum | 78335.4 | 27832.8 | 0.042* | 0.005* | 0.213 | |
| Keratin type II cytoskeletal 75 | Citrate | 17435.1 | 3417.5 | 0.910 | ||
| EDTA | 23954.2 | 5558.9 | 0.231 | 0.258 | ||
| Serum | 39640.8 | 7021.8 | 0.013* | 0.017* | 0.213 | |
| Mannan-binding lectin serine protease 1 | Citrate | 19359.2 | 838.0 | 1.00 | ||
| EDTA | 369877.9 | 451573.1 | 0.089 | 0.005* | ||
| Serum | 82930.6 | 47213.7 | 0.308 | 0.031* | 0.497 | |
| NFX1-type zinc finger-containing protein 1 | Citrate | 1665.6 | 406.6 | 0.258 | ||
| EDTA | 5768.4 | 2119.3 | 0.365 | 0.042* | ||
| Serum | 9485.0 | 2649.1 | 0.07 | 0.003* | 0.365 | |
| Pro-thrombin | Citrate | 16532.6 | 2078.7 | 0.174 | ||
| EDTA | 36920.9 | 16154.9 | 1.00 | 0.174 | ||
| Serum | 92027.3 | 11207.8 | 0.113 | 0.003* | 0.113 | |
| Putative uncharacterized protein MYH16 | Citrate | 393.4 | 93.2 | 0.734 | ||
| EDTA | 5638.6 | 2475.0 | 0.024* | 0.009* | ||
| Serum | 734.2 | 125.3 | 0.308 | 0.174 | 0.213 | |
| Serotransferrin | Citrate | 246039.8 | 45043.8 | 0.428 | ||
| EDTA | 603798.7 | 62743.3 | 0.258 | 0.054 | ||
| Serum | 1127260.8 | 200381.7 | 0.031* | 0.003* | 0.308* | |
| Transthyretin | Citrate | 850.2 | 268.5 | 0.024* | ||
| EDTA | 5663.8 | 5519.4 | 1.00 | 0.024* | ||
| Serum | 1237.8 | 259.7 | 0.113 | 0.497 | 0.113 | |
| Vitamin D-binding protein | Citrate | 11973.0 | 1888.2 | 0.497 | ||
| EDTA | 43094.2 | 16511.6 | 0.258 | 0.07 | ||
| Serum | 79524.5 | 15148.7 | 0.042* | 0.007* | 0.365 | |