Fig 2. Leptospiral LPS avoids TRIF-dependent responses but activates MyD88.

A) Production of KC, RANTES and NO by WT and MyD88 KO BMDMs after 24h stimulation with 500 ng/mL, 1 μg/mL and 2 μg/mL of LPS of L. interrogans (L.i, green) or LPS of E. coli (E.c, blue). B) mRNA levels of RANTES, iNOS and C) IFN-β in RAW264.7 cells after stimulation with 1 μ/mL of LPS of L. interrogans (L.i, green) or LPS of E. coli (E.c, blue). Data are represented as mean (+/- SD) of n = 3/4 technical replicates and are representative of at least 3 independent experiments. Statistical analyses were performed using the non-parametric Mann-Whitney test.