Skip to main content
. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Gen Comp Endocrinol. 2020 Jun 27;296:113543. doi: 10.1016/j.ygcen.2020.113543

Figure 1. Total ion chromatogram of steroid hormones.

Figure 1.

Hormones were extracted from double charcoal stripped, delipidized human serum, using MTBE after spiking with internal standards and injected into the LC-MS/MS. Fourteen hormones were chromatographically separated from a high concentration of quality control sample (5 to 125 ng/mL depends on the hormone) using a C18 column in 12 min. A binary gradient was applied with mobile phase A containing 0.1 mM ammonium fluoride in water and mobile phase B, 100% methanol. The instrument was run under the scheduled MRM method.