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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Gen Comp Endocrinol. 2020 Jun 27;296:113543. doi: 10.1016/j.ygcen.2020.113543

Figure 2: Relative intensities of 17β-estradiol (E2) peaks with or without derivatization.

Figure 2:

E2 (0.1 ng/mL) was spiked into double charcoal stripped, delipidized human serum and extracted using MTBE. The E2 peak was acquired in negative ion mode (A) and then the remainder of the sample was dried, reconstituted in sodium bicarbonate buffer, derivatized using dansyl chloride (B) and reanalyzed by LC-MS/MS in positive ion mode.