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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Gen Comp Endocrinol. 2020 Jun 27;296:113543. doi: 10.1016/j.ygcen.2020.113543

Figure 3. Effect of peak smoothing on separation of estrone (E1) and 17β-estradiol (E2).

Figure 3.

Calibration samples were prepared by spiking working solution into serum followed by serially dilution and analyzing with LC-MS/MS. Peaks of E1 and E2 in the ranges of 1 to 0.006 ng/mL were selected and smoothing value of 0 (A) and 2 (B) were compared in MultiQuant software.