Table 1:
List of steroid hormones, MRMs and settings for data acquisition.
| Hormone* | Parental ions (m/z) | Product ions (m/z) | Retention Time (min) | DP (volts) | CE (volts) | CPX (volts) |
|---|---|---|---|---|---|---|
| Corticosterone | 347 | 121/105 | 8.4 | 80 | 32 | 12 |
| Cortisol | 363 | 309/121 | 7.2 | 80 | 32 | 9 |
| 11-Ketotestosterone | 303 | 259/121 | 7.4 | 60 | 34 | 12 |
| Progesterone | 315 | 109/97 | 10.8 | 80 | 32 | 14 |
| Testosterone | 289 | 109/97 | 9.5 | 100 | 27 | 12 |
| 17OH-Progesterone | 331 | 109/97 | 9.8 | 90 | 28 | 9 |
| Aldosterone | 361 | 343/315 | 5.8 | 90 | 25 | 23 |
| Dihydrotestosterone | 291 | 255/91 | 10.5 | 80 | 21 | 16 |
| Levonorgestrel | 313 | 245/109 | 9.9 | 130 | 25 | 12 |
| Equilin | 267 | 223/143 | 9.0 | −105 | −44 | −15 |
| Estrone | 269 | 145/143 | 9.2 | −130 | −48 | −17 |
| Estradiol | 271 | 183/145 | 9.5 | −140 | −56 | −20 |
| Estriol | 287 | 171/145 | 5.2 | −120 | −49 | −17 |
| Ethinylestradiol | 295 | 145/143 | 9.4 | −130 | −56 | −20 |
*
Two transitions were considered for each hormone, one for quantitation and the second for qualification. DP, Declustering potential; CE, Collision energy; CPX, Collision cell exit potential.