Table 2.

Calibration curve regression equations and lower limit of quantification (LLOQ) of steroid hormones in high and low volume plasma.

Hormone	Regression equation		LLOD (ng/ml)		LLOQ (ng/ml)
	10 μL	245 μL	10 μL	245 μL	10 μL	245 μL
Corticosterone	y=0.0024x+3.35e–4 (r = 0.998)	y=0.028x+0.001 (r = 0.997)	0.5	0.025	0.5	0.05
Cortisol	y=0.013x+0.033 (r = 0.999)	y=0.124x+0.012 (r = 0.999)	0.012	0.006	0.025	0.006
11-KT	y=0.014x+3.156e–4 (r = 1)	y=0.153x+0.0102 (r = 0.998)	0.025	0.012	0.05	0.012
Progesterone	y=0.035x–0.0012 (r = 1)	y=0.403x+0.0027 (r = 1)	0.012	0.0003	0.012	0.0003
Testosterone	y=0.016x–2.17e–4 (r = 0.998)	y=0.114x-4.01e–4 (r = 0.999)	0.012	0.0006	0.012	0.001
17OH-Progesterone	y=0.011x+4.91e–5 (r = 0.999)	y=0.119x+1.84e–4 (r = l)	0.012	0.001	0.012	0.001
Aldosterone	y=0.0098x–8.72e–4 (r = l)	y=0.131x+0.0015 (r = 0.999)	0.05	0.005	0.05	0.005
Dihydrotestosterone	y=0.013x–7.87e–5 (r = l)	y=0.116x+6.76e–4 (r = 0.999)	0.125	0.005	0.125	0.012
Levonorgestrel	y=0.018x+3.456e–4 (r = 1)	y=0.104x+4.39e–4 (r = 1)	0.025	0.012	0.05	0.012
Equilin	y=0.0061x+3.76e–4 (r = 0.997)	y=0.02x–2.597e–4 (r = l)	0.05	0.003	0.05	0.012
Estrone	y=0.0062 x+0.025 (r = 0.999)	y=0.067x+0.0278 (r = l)	0.012	0.006	0.025	0.006
Estradiol*	y=0.0016x–8.03e–4 (r = 0.999)	y=0.017x+3.57e–4 (r = 0.998)	0.05	0.006	0.25	0.025
Estriol	y=9.14e–4 x+3.3e–5 (r = 0.999)	y=0.007x+7.16e–5 (r = l)	0.125	0.025	0.25	0.025
Ethinylestradiol	y=0.0011x+2.5e–5 (r = l)	y=0.012x+1.77e–5 (r = 1)	0.125	0.007	0.125	0.015

^*Data are based on the quantification of derivatized Estradiol.