Table 4.

Comparison of hormone quantification in LMB using 245 and 10 μL plasma (ng/mL).

Hormones	Male		Female
	245 μL	10 μL	245 μL	10 μL
Cortisol	41.1±3.9	43.5±4.9	91.6±21.1*	104.3±21.4*
11-KT	0.12±0.05**	0.15±0.06**	-	-
Testosterone	0.1+0.03	0.14±0.04	0.06±0.02	0.08±0.04
E2#	0.87±0.2	3.1±1.5	1.7±0.8	3±1.3

Data are mean of 5 plasma samples ±SE. No peaks were observed in female for 11-KT and the concentration can be considered as zero.

^*Indicate significant differences between the calculated values (p ≤0.05 and p ≤0.01, respectively) for males and females measured in similar volumes.

^**Indicate significant differences between the calculated values (p ≤0.05 and p ≤0.01, respectively) for males and females measured in similar volumes.

^#:Only 10 μL plasma samples were derivatized using dansyl chloride.