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. Author manuscript; available in PMC: 2021 Sep 15.
Published in final edited form as: Gen Comp Endocrinol. 2020 Jun 27;296:113543. doi: 10.1016/j.ygcen.2020.113543

Table 5.

Detection limits and calibration curve equations in LC-MS/MS and RIA techniques.

LC-MS/MS Radioimmunoassay
T ng/mL E2 ng/mL T ng/mL E2 ng/mL
Calibration range 0.0025 to 20 0.03 to 100 0.20 to 250 0.10 to 100
LRE (R2) y = 0.9961x +0.0135 (1) y = 1.0801x + 0.0367 (0.999) y = −0.4584x + 1.0671 (0.996) y = −0.4671x + 1.3556 (0.995)

T, testosterone; E2, 17β-estradiol; LRE, Linear Regression equation; R2, correlation coefficient