Figure 6 – Type I, but not type III IFNs promote immune cell recruitment into the lung.

(A) Intersection of IFNβ and IFNλ3 responsive genes in PH5CH8 WT cells. (B) Predicted activation state of kinases significantly associated transcriptional changes after IFNβ or IFNλ3 treatment using IPA. Color indicates predicted activation (purple)or predicted inhibition (green). (C) Predicted activation state of transcription factors found to be significantly associated with transcriptional changes after IFNβ or IFNλ3 treatment using IPA. Color indicates predicted activation (blue) or predicted inhibition (brown). (D) Bubble plot representation of significantly enriched biological functions in IFN-treated cells using IPA. Bubble color represents activation z-scores and bubble size represents the -log₁₀ p-value of enrichment. Statistical significance was determined by an activation z-score > |2| and a -log₁₀ p-value > 1.32, which correspond to a p-value of 0.05. Increases in -log₁₀ p-value are indicative of increased statistical significance. (E) Immunoblot analysis of IRF1 in A549 cells treated with IFNβ (25 IU/ml) or IFNλ3 (100 ng/ml) over time. (F) Quantification of pulmonary expression of Cxcl10, Oas1a, and Isg15 mRNA following inoculation with murine IFNβ (2μg) or murine IFNΑ3 (4μg) relative to Actin control. (G) Quantification of immune cells in BAL of IFN-treated mice at 48h post-treatment. Unless otherwise indicated, data is representative of mean ± SEM of 3 independent experiments. See also Figure S6.