Table 1.
Comparison of kinetics parameters and dimer dissociation constants.
| Enzymes | Kinetics parametersa |
Dimer dissociation constants Kd (μM) | ||
|---|---|---|---|---|
| Km (μM) | kcat (s−1) | kcat/Km (s−1μM−1) | ||
| 2019-nCoV Mpro | 27.56 ± 4.94 | 163.69 ± 15.09 | 5.94 ± 3.05 | 1.23 |
| SARS-nCoV Mpro | 25.83 ± 4.20 | 76.48 ± 6.04 | 2.96 ± 1.44 | 0.35 |
a
All the experiments were performed in triplicates. Km, Machaelis-Menten constant; kcat, catalytic constant; kcat/Km, specificity constant for hydrolysis of peptide substrate. (Abz-TSAVLQSGFRK-Dnp).