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. 2020 Aug 10;46(4):1477–1489. doi: 10.3892/ijmm.2020.4699

Figure 2.

Figure 2

Knockdown of FOXD2-AS1 suppresses cell proliferation, migration and invasion in breast cancer. (A and B) Following transfection with shRNA-FOXD2-AS, the mRNA level of FOXD2-AS1 was measured by RT-qPCR in MCF-7 and MDA-MB-468 cells. (C and D) CCK-8 assay was performed to determine cell proliferation following transfection. (E-G) Cell cycle distribution was determined and analyzed by FACS. (H and I) The protein expression of cyclin E1, CDK2 and p21 was determined by western blot analysis. Knockdown of FOXD2-AS1 suppresses cell proliferation, migration and invasion in breast cancer. (J) Wound healing assay was performed to detect the migration of both MCF-7 and MDA-MB-468 cells. (K and L) Relative migration rate of MCF-7 and MDA-MB-468 cells was quantified, respectively. (M) Transwell assay was performed to detect the invasion of both MCF-7 and MDA-MB-468 cells. (N and O) Relative cell invasive rate of MCF-7 and MDA-MB-468 cells was quantified, respectively. (P) MMP-2 and MMP-9 protein expression in MCF-7 transfected with shRNA-FOXD2-AS1 was detected and quantified. (Q) MMP-2 and MMP-9 protein expression in MDA-MB-468 transfected with shRNA-FOXD2-AS1 was detected and quantified. *P<0.05, **P<0.01 and ***P<0.001, vs. shRNA-NC. CDK2, cyclin-dependent kinase-2; MMP, matrix metalloproteinase.