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. 2020 Aug 10;46(4):1477–1489. doi: 10.3892/ijmm.2020.4699

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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S100A1 is upregulated in breast cancer cells. (A) The mRNA level of S100A1 in MCF-10A and human breast cancer cell lines (MCF-7, MDA-MB-468, MDA-MB-453 and BT-549) was determined by RT-qPCR. ^*P<0.05, ^**P<0.01 and ^***P<0.001, vs. MCF-10A cells. (B and C) In shRNA-FOXD2-AS1-trans-fected MCF-7 and MDA-MB-468 cells, the protein expression of Sl00A1 was detected by western blot analysis. ^*P<0.05 and ^***P<0.001, vs. shRNA-NC. (D and E) Following transfection with pcDNA-FOXD2-AS1, the mRNA level of FOXD2-AS1 in MCF-7 and MDA-MB-468 cells was detected by RT-qPCR. ^**P<0.01 and ^***P<0.001, vs. pcDNA-NC. MCF-7 and MDA-MB-468 cells were transfected with shRNA-S100A1, and the protein expression and mRNA level of S100A1 in (F and G) MCF-7 and (H and I) MDA-MB-468 cells were determined by western blot analysis and RT-qPCR, respectively. ^**P<0.01 and ^***P<0.001, vs. shRNA-NC. S100A1, S100 calcium binding protein A1.