Figure 7.

Knockdown of FOXD2-AS1 suppresses tumor progression of breast cancer in vivo. (A) Each mouse was injected with 1.0×10⁷ MDA-MB-468 cells, which were stably transfected with either shRNA negative control or shRNA-FOXD2-AS1, subcutaneously at the right axillary lymph node. Following sacrifice, the tumors were excised and photographed. (B and C) During the experiment, the mouse weight and tumor volume (tumor volume =1/2 × length × width²) was monitored every 5 days until the mice were sacrificed. (D) Protein expression levels of p-YAP, YAP, p-LATS1 and LATS1 in the extracted tumors were determined by western blot analysis and were then quantified. ^***P<0.001, vs. shRNA-NC. LATS1, large tumor suppressor homolog 1; YAP, yes-associated protein.