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. 2020 Aug 3;46(4):1321–1334. doi: 10.3892/ijmm.2020.4692

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Copyright: © Lyu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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In vitro assays of NRP1 functions in the Ealy926 and MVEC cell lines with overexpression or knockdown of NRP1. (A) Efficient NRP1 overexpression or knockdown was confirmed using western blotting. GAPDH was used as the loading control. (B) Proliferative rates of Ealy926 and MVEC cell lines with overexpression or knockdown of NRP1, detected using CCK-8 assays. (C) Apoptosis assays with Ealy926 and MVEC cell lines after NRP1 overexpres-sion and knockdown as determined using flow cytometry. (D) Migration (×200) and (E) in vitro tube-forming activity (×40) of Ealy926 and MVEC cells after overexpression or knockdown of NRP1. The number of cells or tubes were counted in five randomly selected fields. NC represents the control group and NRP1 represents the NRP1 overexpression group. ^*P<0.05 and ^**P<0.01 vs. NC. NRP1, neuropilin 1; siNRP1, siRNA knockdown of the NRP1 group; CCK-8, Cell Counting Kit-8.