Effects of BANCR inhibition on the proliferation, migration, invasion and EMT of ESCC cells. (A) The expression of BANCR in ESCC and HET-1A cells was measured by RT-qPCR. (B-H) KYSE450 and KYSE510 cells were transfected with si-NC, si-BANCR-1, or si-BANCR-2. (B) The expression of BANCR in KYSE450 and KYSE510 cells was examined by RT-qPCR. (C and D) The proliferation of KYSE450 and KYSE510 cells was evaluated by MTT assay. (E and F) Transwell assay was conducted to determine the (E) migration and (F) invasion of KYSE450 and KYSE510 cells. (G and H) Western blotting analysis of the expression levels of E-cadherin, N-cadherin, and Vimentin in KYSE450 and KYSE510 cells. Data are presented as the means ± standard error of 3 experimental results. One-way ANOVA was applied to compare differences between groups. *P<0.05. BANCR, BRAF activated non-coding RNA; ESCC, esophageal squamous cell carcinoma.