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. 2020 Jul 27;46(4):1311–1320. doi: 10.3892/ijmm.2020.4684

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Copyright: © Fan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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TUSC8 functions as a sponge of miR-197-3p. (A) FISH staining revealed that TUSC8 was mainly distributed in the cytoplasm. (B) RT-qPCR was employed to detect the level of candidate miRNAs in the osteoblastic cell line, OB3, and in OS cell lines (MG-63, U2OS, Saos-2 and HOS). (C) miR-197-3p expression in OS cells was enhanced by transfection of miR-197-3p mimic. (D and E) Luciferase reporter and RNA immunoprecipitation (RIP) assays were carried out to confirm the interaction between miR-197-3p and TUSC8. (F) RT-qPCR was performed to measure miR-197-3p expression in OS tissues and adjacent normal tissues. ^*P<0.05 vs. NC mimics or IgG.