Figure 6.

ICS II activates PI3K/Akt signaling in H9c2 cells via the upregulation of miR-7-5p. (A) and (B) H9c2 cells were transfected as indicated, and total cellular lysates were subjected to western blot analysis. H9c2 cells were treated as indicated, then total cellular lysates were subjected to western blot analysis. (C) Cell viability, (D) apoptosis and (E) LDH release were assayed and (F) total cellular lysates were subjected to western blot analysis. (G) migration and invasiveness of cells were also assayed. Data are presented as the mean ± SD of three independent experiments. ^*P<0.05, ^**P<0.01 and ^***P<0.001. ICS II, Icariside II; miR/miRNA, micro RNA; NC, negative control; LDH, lactate dehydrogenase; Ctrl, control.