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. 2020 Jul 28;46(4):1525–1537. doi: 10.3892/ijmm.2020.4688

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Copyright: © Zhou et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of Ac on mitochondrial membrane potential and colorectal cancer cell apoptosis. (A) AC treatment for 24 h decreased mitochondrial membrane potential in HCT116 cells, where the apoptosis of CRC cells was determined by detecting the intensity of green (low) fluorescence from red (high) fluorescence. (B) Quantification of the mitochondrial membrane potential. (C) The apoptosis of HCT116 cells treated with various concentrations of AC for 24 h was measured by flow cytometry and (D) statistically analyzed. (E) The apoptosis of HCT116 cells treated with various concentrations of AC for 72 h was detected by flow cytometry and (F) statistically analyzed. Data are expressed as mean ± standard deviation from three independent experiments. ^*P<0.05 vs. control cells. AC, asiaticoside; RF, red fluorescence; GF, green fluorescence; PI, propidium iodide; AV, Annexin-V.