Figure 6.

Effect of AC on the NF-κB signaling pathway. (A) mRNA and (B) protein expression levels of the NF-κB signaling pathway-related molecules IκBα, p-IκBα, P65 and p-P65 in HCT116 cells treated with AC for 72 h. (C) Quantification of the western blots. (D) Nuclear translocation of P65 detected by western blotting in HCT116 cells treated with 2 µM AC for 72 h. (E) Quantification of the western blots. Data are expressed as mean ± standard deviation from three independent experiments. ^*P<0.05 vs. control cells. AC, asiaticoside; p-, phosphorylated.