Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Tie2 (C-20) (Rabbit polycloned IgG) | Santa Cruz | Cat#: SC-324 | WB: 1:1000 |
| Antibody | GAPDH (Rabbit polycloned IgG) |
Santa Cruz | Cat#: SC-25778 | WB: 1:1000 |
| Antibody | β-Tubulin (Rabbit polycloned IgG) |
Santa Cruz | Cat#: SC-9104 | WB: 1:1000 |
| Antibody | RAT anti Mouse Ly-6B.2 ALLOANTIGEN | Bio-Rad | Cat#: MCA771G | IF:1:200 |
| Antibody | CD144 (VE-cadherin) | BD Biosciences | Cat#: 555661 | IF:1:100 |
| Antibody | Human IgG2 isotype control | BioXcell | Cat# BE0301 | |
| Antibody | Anti-MMP14 (E2C6) | Botkjaer et al., 2016 | PMID:26934448 | A kind gift from Prof. Gillian Murphy and Dr. Yoshifumi Itoh |
| Commercial assay or kit | Human Tie-2 DuoSet | R and D | Cat#: DY5159 | |
| Commercial assay or kit | Mouse Tie-2 Quantikine ELISA Kit | R and D | Cat#: MTE200 | |
| Commercial assay or kit | CBA Mouse Inflammation Kit | BD | Cat#: 552364 | |
| Peptide, recombinant protein | Recombinant Human TNF-alpha | R and D | Cat#: 210-TA-020 | |
| Peptide, recombinant protein | Recombinant Human MMP14 (catalytic domain) |
Enzo | Cat#: ALX-201–098 C010 | |
| Peptide, recombinant protein | Recombinant Human TIE2 protein | Sino Biological | Cat#: 10700-H03H | |
| Software, algorithm | ImageJ | NIH, Bethesda, MD (Version 1.52P) |
https://imagej.nih.gov/ij/ | |
| Software, algorithm | Prism8 | Graphpad | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | Flowjo | BD | https://www.flowjo.com/solutions/flowjo/downloads | |
| Other | LPS Escherichia coli serotype O111:B4 |
Sigma-Aldrich | Cat#: L4130 |