Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK GnTi | ATCC | CRL-3022 | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200-MBP | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-Atg13 | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-ATG101 | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG- MBP-TSF-TEVcs-ULK1 |
This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG- EGFP-ATG13 |
This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG- GST-TEVcs-EGFP-ATG101 |
This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG- GST-TEVcs-EGFP-FIP200-MBP |
This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pGST2-NDP52 | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-MBP-NDP52 | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pGEX5-4xUb | Zaffagnini et al., 2018 | From Sascha Martens group (Vienna) | |
| Recombinant DNA reagent | pGST2-NDP52-mCherry | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-TSF-NDP52-mCherry | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pET-6xHis-TEVcs-NDP52 | This paper | From Sascha Martens group (Vienna) | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200(1274 C) | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200(N-640)-MBP | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200(636-C)-MBP | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200(delta790-1050, ΔMR)-MBP | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200(1363-1370Mut, ΔNDP52)-MBP | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Recombinant DNA reagent | pCAG-GST-TEVcs-FIP200(1274 C) M1-M8 | This paper | See ‘plasmid construction’ section. Can be obtained from the Hurley lab. | |
| Commercial assay or kit | ADP-Glo Max Assay | Promega, Madison, WI | V6930 | |
| Software, algorithm | Proteome Discoverer 2.1 | Thermo Fisher Scientific, Waltham, MA | https://www.thermofisher.com/order/catalog/product/OPTON-30795 | |
| Software, algorithm | HDExaminer | Sierra Analytics, Modesto, CA | http://massspec.com/hdexaminer/ | |
| Software, algorithm | Nikon Elements microscope imaging software 4.60 | Nikon Corporation, Tokyo, Japan | https://www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer | |
| Software, algorithm | Custom Python scripts and Jupyter notebooks | This paper | Access at https://github.com/Hurley-Lab/FIP-NDP52-paper | |
| Software, algorithm | Relion | SCR_016274 | ||
| Other | Glutathione Sepharose 4B GST-tagged protein purification resin | GE healthcare, Chicago, IL | Cat#17075605 | |
| Other | Amylose Resin | New England Biolabs, Ipswich, MA | Cat#E8021L | |
| Other | Strep-Tactin Superflow high capacity 50% suspension | IBA Lifesciences, Göttingen, Germany |
Cat# 2-1208-010 |