Table 3. Complex I subunit homologues in plants, mammals, yeast and bacteria.
V. radiata homologues were obtained by performing BLASTp searches of the Arabidopsis thaliana genes (Meyer et al., 2019; Braun et al., 2014). Mammalian, yeast and bacterial homologues were obtained from Letts and Sazanov, 2015. Additional BLASTp searches were performed wherever necessary. Given the high sequence similarity between the carbonic anhydrase (CA) paralogues, the names of the V. radiata CA proteins appear to have been mis-assigned in the genetic databases relative to their A. thaliana homologues. The CA1, CA2, CA2-like nomenclature used in the table is the one that, based on our sequence alignments, best represents homology to the A. thaliana CA proteins. N, NADH-binding module; Q, quinone-binding module; PP, proximal-pumps module; PD, distal-pumps module; CA, carbonic anhydrase domain.
| Module | Vigna radiata protein name | Vigna radiata gene | Vigna radiata uniprot identifier | Arabidopsis thaliana protein name | Arabidopsis thaliana gene | Homo sapiens name | Ovis aries name | Mus musculus name | Yarrowia lipolytica name | Thermus thermophilus name |
|---|---|---|---|---|---|---|---|---|---|---|
| CORE peripheral arm | ||||||||||
| N | NDUS1 | LOC106757688 | A0A1S3TQ85 | 75 kDa | At5g37510 | NDUFS1 | NDUFS1 | NDUFS1 | NUAM | Nqo3 |
| N | NDUV1 | LOC106772405 | A0A1S3V7V2 | 51 kDa | At5g08530 | NDUFV1 | NDUFV1 | NDUFV1 | NUBM | Nqo1 |
| N | NDUV2 | LOC106762461 | A0A1S3U769 | 24 kDa | At4g02580 | NDUFV2 | NDUFV2 | NDUFV2 | NUHM | Nqo2 |
| Q | NDUS2 | nad7 | E9KZN6 | Nad7 | AtMg00510 | NDUFS2 | NDUFS2 | NDUFS2 | NUCM | Nqo4 |
| Q | NDUS3 | nad9 | E9KZM7 | Nad9 | AtMg00070 | NDUFS3 | NDUFS3 | NDUFS3 | NUGM | Nqo5 |
| Q | NDS7 | LOC106762764 | A0A1S3U8J5 | PSST | At5g11770 | NDUFS7 | NDUFS7 | NDUFS7 | NUKM | Nqo6 |
| Q | NDS8 | LOC106775047 | A0A1S3VGS8 | TYKY | At1g79010, At1g16700 | NDUFS8 | NDUFS8 | NDUFS8 | NUIM | Nqo9 |
| CORE membrane arm | ||||||||||
| PP | NU1M | nad1 | A0A1S4ETV6 | Nad1 | AtMg00516, AtMg01120, AtMg01275 | MT-ND1 | MT-ND1 | MT-ND1 | NU1M | Nqo8 |
| PP | NU2M | nad2 | E9KZK9 | Nad2 | AtMg00285, AtMg01320 | MT-ND2 | MT-ND2 | MT-ND2 | NU2M | Nqo14 |
| PP | NU3M | nad3 | Q9XPB4 | Nad3 | AtMg00990 | MT-ND3 | MT-ND3 | MT-ND3 | NU3M | Nqo7 |
| PP | NU4LM | nad4L | A0A1S4ETY3 | Nad4L | AtMg00650 | MT-ND4L | MT-ND4L | MT-ND4L | NULM | Nqo11 |
| PP | NU6M | nad6 | E9KZM5 | Nad6 | AtMg00270 | MT-ND6 | MT-ND6 | MT-ND6 | NU6M | Nqo10 |
| PD | NU4M* | nad4 | E9KZL8 | Nad4 | AtMg00580 | MT-ND4 | MT-ND4 | MT-ND4 | NU4M | Nqo13 |
| PD | NU5M* | nad5 | E9KZL1 | Nad5 | AtMg00060, AtMg00513, AtMg00665 | MT-ND5 | MT-ND5 | MT-ND5 | NU5M | Nqo12 |
| ACCESSORY membrane arm | ||||||||||
| PP | NDUA1 | LOC106758834 | A0A1S3TU57 | MWFE | At3g08610 | NDUFA1 | NDUFA1 | NDUFA1 | NIMM | - |
| PP | NDUA3 | LOC106754061 | A0A1S3TCK0 | B9 | At2g46540 | NDUFA3 | NDUFA3 | NDUFA3 | NI9M | - |
| PP | NDUA8-B | LOC106778955 | A0A1S3VVN6 | PGIV | At3g06310, At5g18800 | NDUFA8 | NDUFA8 | NDUFA8 | NUPM | - |
| PP | NDUA13-A | LOC106769964 | A0A1S3UYW0 | B16.6 | At2g33220, At1g04630 | NDUFA13 | NDUFA13 | NDUFA13 | NB6M | - |
| PP | NDUC1 | LOC106771273 | A0A1S3V2Z3 | - | - | NDUFC1 | NDUFC1 | NDUFC1 | - | - |
| PP | NDUS5 | LOC106757655 | A0A1S3TQ33 | 15 kDa | At3g62790, At2g47690 | NDUFS5 | NDUFS5 | NDUFS5 | NIPM | - |
| PP | NDUB8 | LOC106765859 | A0A1S3UJ95 | ASHI | At5g47570 | NDUFB8 | NDUFB8 | NDUFB8 | NIAM | - |
| PP | NDUB10-B | LOC106774903 | A0A1S3VGT1 | PDSW | At1g49140, At3g18410 | NDUFB10 | NDUFB10 | NDUFB10 | NIDM | - |
| PP | NDUA11* | LOC106756741 | A0A1S3TLY8 | B14.7 | At2g42210 | NDUFA11 | NDUFA11 | NDUFA11 | NUJM | - |
| Module | Vigna radiata protein name | Vigna radiata gene | Vigna radiata Uniprot identifier | Arabidopsis thaliana protein name | Arabidopsis thaliana gene | Homo sapiens name | Ovis aries name | Mus musculus name | Yarrowia lipolytica name | Thermus thermophilus name |
| ACCESSORY membrane arm | ||||||||||
| PD | NDUB1* | LOC106775330 | A0A1S3VI15 | MNLL | At4g16450 | NDUFB1 | NDUFB1 | NDUFB1 | - | - |
| PD | NDUC2* | LOC106767534 | A0A1S3UPL8 | B14.5b | At4g20150 | NDUFC2 | NDUFC2 | NDUFC2 | NEBM | - |
| PD | NDUB2* | LOC106754955 | A0A1S3TFG6 | AGGG | At1g76200 | NDUFB2 | NDUFB2 | NDUFB2 | - | - |
| PD | NDUB3* | LOC106769121 | A0A1S3UVV0 | B12 | At2g02510, At1g14450 | NDUFB3 | NDUFB3 | NDUFB3 | NB2M | - |
| PD | NDUB4* | LOC106766640 | A0A1S3ULL3 | B15 | At2g31490 | NDUFB4 | NDUFB4 | NDUFB4 | NB5M | - |
| PD | NDUB5* | LOC106767179 | A0A1S3UND4 | - | - | NDUFB5 | NDUFB5 | NDUFB5 | NUNM | - |
| PD | NDUB7* | LOC106770979 | A0A1S3V2B8 | B18 | At2g02050 | NDUFB7 | NDUFB7 | NDUFB7 | NB8M | - |
| PD | NDUB9* | LOC106760947 | A0A1S3U1J6 | B22 | At4g34700 | NDUFB9 | NDUFB9 | NDUFB9 | NI2M | - |
| PD | NDUB11* | LOC106771273 | A0A1S3V2Z3 | ESSS | At2g42310, At3g57785 | NDUFB11 | NDUFB11 | NDUFB11 | NESM | - |
| ACCESSORY peripheral arm | ||||||||||
| N | NDUA2 | LOC106759195 | A0A1S3TVC7 | B8 | At5g47890 | NDUFA2 | NDUFA2 | NDUFA2 | NI8M | - |
| N | NDUA12 | LOC106776991 | A0A1S3VNK7 | B17.2 | At3g03100 | NDUFA12 | NDUFA12 | NDUFA12 | N7BM | - |
| N | NDUS4 | LOC106765762 | A0A1S3UIW7 | 18 kDa | At5g67590 | NDUFS4 | NDUFS4 | NDUFS4 | NUYM | - |
| N | NDUS6 | LOC106779709 | A0A1S3VYF3 | 13 kDa | At3g03070 | NDUFS6 | NDUFS6 | NDUFS6 | NUMM | - |
| Q | NDUA5 | LOC106760411 | A0A1S3U023 | B13 | At5g52840 | NDUFA5 | NDUFA5 | NDUFA5 | NUFM | - |
| Q | NDUA6† | LOC106780789 | A0A1S3W1K8 | B14 | At3g12260 | NDUFA6 | NDUFA6 | NDUFA6 | NB4N | - |
| Q | NDUA7 | LOC106768957 | A0A1S3UVC7 | B14.5a | At5g08060 | NDUFA7 | NDUFA7 | NDUFA7 | NUZM | - |
| Q | NDUA9 | LOC106772694 | A0A1S3V8W7 | 39 kDa | At2g20360 | NDUFA9 | NDUFA9 | NDUFA9 | NUEM | - |
| Plant-specific accessory | ||||||||||
| CA | CA1‡ | LOC106778103 | A0A1S3VT00 | Gamma-CA 1 | At1g19580 | - | - | - | - | - |
| CA | CA2§ | LOC106761992, LOC106761993 | A0A1S3U566, A0A1S3U544 | Gamma-CA 2 | At1g47260 | - | - | - | - | - |
| CA | CA2-L¶ | LOC106765552 | A0A1S3UI49 | Gamma CA-like 2 | At3g48680 | - | - | - | - | - |
| CA | CA3* | n.a.** | n.a.** | Gamma-CA 3 | At5g66510 | - | - | - | - | - |
| CA | CA1-L* | n.a.** | n.a.** | Gamma-CA-like 1 | At5g63510 | - | - | - | - | - |
| PP | NDUX1†† | LOC106775330 | A0A1S3VI15 | 20.9 kDa | At4g16450 | - | - | - | NUXM | - |
| PP | P2/16 kDA | LOC106755236 | A0A1S3TGE7 | P2 | At2g27730 | - | - | - | - | - |
| Plant-specific accessory | ||||||||||
| Unconfirmed plant CI subunits (not seen in CI*) | MICOS (DUF543) | LOC106779628 | A0A1S3VY06 | MICOS subunit Mic10 | At1g72165 | - | - | - | - | - |
| Uncharacterized protein LOC106758628 | LOC106758628 | A0A1S3TTD7 | NDU10 | At4g00585 | - | - | - | - | ||
| P1/11 kDA | LOC106761134 | A0A1S3U2B9 | P1 | At1g67350 | - | - | - | - | - | |
| P3 | LOC106755586 | A0A1S3THM0 | P3 | At5g14105 | - | - | - | - | - | |
| P4 | LOC106767179 | A0A1S3UND4 | P4 | At1g67785 | - | - | - | - | - | |
| TIM22−4 × 1 | LOC106779665 | A0A3Q0EN44 | TIM22-4 | At1g18320 | - | - | - | - | - | |
| TIM22−4 × 2 | LOC106779665 | A0A1S3VZ08 | TIM22-1 | At3g10110 | - | - | - | - | - | |
| TIM23-2 | LOC106761237 | A0A1S3U2K1 | TIM23-2 | At1g72750 | - | - | - | - | - | |
| Uncharacterized protein LOC106768488 isoform X4 | LOC106768488 | A0A1S3UST2 | SH3/FCH domain protein | At1g68680 | - | - | - | - | - | |
| UDP-galactose transporter 1 | LOC106762681 | A0A1S3U838 | TPT domain-containing protein | At1g72180 | - | - | - | - | - | |
| Gravitropic in the light 1 | LOC106779790 | A0A1S3VYR1 | DUF641 domain-containing protein | At2g28430 | - | - | - | - | - | |
*Not seen in CI*.
†Only the C-terminus seen in CI* (see Main body and Discussion).
‡Called gamma carbonic anhydrase one in Uniprot.
§Called gamma carbonic anhydrase 1, mitochondrial in Uniprot (mis-assigned in the database).
¶Called gamma carbonic anhydrase-like 2, mitochondrial in Uniprot (mis-assigned in the database).
**Homologue not found using BLASTp.
††New identified subunit.