Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | w1118 | Bloomington Drosophila Stock Center | BDSC 5905 | isogenic |
| Genetic reagent (D. melanogaster) | Canton-S | O. Shafer lab | n/a | WT |
| Genetic reagent (D. melanogaster) | Oregon-R | C. Collins lab | n/a | WT |
| Genetic reagent (D. americana) | Drosophila americana | P. Wittkopp lab | n/a | Non melanogaster Drosophila |
| Genetic reagent (D. mauritiana) | Drosophila mauritiana | P. Wittkopp lab | n/a | Non melanogaster Drosophila |
| Genetic reagent (D. melanogaster) | glass60J | O. Shafer lab | n/a | Mutant for glass |
| Genetic reagent (D. melanogaster) | w;nSyb-GAL4/Cyo | M. Dus lab | n/a | pan-neuronal |
| Genetic reagent (D. melanogaster) | w;+;nSyb-GAL4 | M. Dus lab | n/a | pan-neuronal |
| Genetic reagent (D. melanogaster) | w;UAS-nGFP;Repo-GAL4, tubulin GAL80TS | Buttitta lab stocks | n/a | pan-glial |
| Genetic reagent (D. melanogaster) | w;UAS-nGFP | Buttitta lab stocks | n/a | UAS nuclear GFP |
| Genetic reagent (D. melanogaster) | w;+;UAS-nGFP | Buttitta lab stocks | n/a | UAS nuclear GFP |
| Genetic reagent (D. melanogaster) | w;Moody-GAL4 | C. Collins lab via Klambt Lab | n/a | Sub-perineurial glia |
| Genetic reagent (D. melanogaster) | y,w;mz19-mCD8::GFP | Bloomington Drosophila Stock Center | BDSC 23300 | Antennal lobe projection neuron |
| Genetic reagent (D. melanogaster) | w1118;ELAV-GAL4,UAS-nGFP | Bloomington Drosophila Stock Center | BDSC 49226 | pan-neuronal |
| Genetic reagent (D. melanogaster) | y,w;breathless-GAL4 | DGRC Kyoto | 105276 | Trachea |
| Genetic reagent (D. melanogaster) | w-;GAD1-GAL4/SM6 | O. Shafer lab | n/a | GABAergic |
| Genetic reagent (D. melanogaster) | w-;OK371-GAL4,UASn-GFP | Buttitta lab stocks | n/a | Glutamatergic |
| Genetic reagent (D. melanogaster) | w;ChaT-GAL4 | O. Shafer lab | n/a | Cholinergic |
| Genetic reagent (D. melanogaster) | w1118;+;GMR-12C11-GAL4 | Bloomington Drosophila Stock Center | BDSC 76324 | Tm3a |
| Genetic reagent (D. melanogaster) |
w1118;+;GMR-
42H01-GAL4 |
Bloomington Drosophila Stock Center | BDSC 48150 | Dm9 |
| Genetic reagent (D. melanogaster) | w1118;+;GMR-23G11-GAL4 | Bloomington Drosophila Stock Center | BDSC 49043 | Dm4 |
| Genetic reagent (D. melanogaster) | w1118;+;GMR-30B06-GAL4 | Bloomington Drosophila Stock Center | BDSC 47529 | Dm10 |
| Genetic reagent (D. melanogaster) | w1118;+;GMR-26H07-GAL4 | Bloomington Drosophila Stock Center | BDSC 49204 | Dm2 |
| Genetic reagent (D. melanogaster) | y;w;NP3233-GAL4/Cyo | DGRC Kyoto | 113173 | Astrocyte-like |
| Genetic reagent (D. melanogaster) | y;w;NP2222-GAL4/Cyo | DGRC Kyoto | 112830 | Cortex glia |
| Genetic reagent (D. melanogaster) | w;mz97-GAL4 | C. Collins lab via Klambt Lab | n/a | Wrapping glia |
| Genetic reagent (D. melanogaster) | y,w,UAS-mCD8::RFP,LexAop2-mCD8::GFP; CoinFLP-LexA::GAD.GAL4 | Bloomington Drosophila Stock Center | BDSC 59270 and 59271 | CoinFLP |
| Genetic reagent (D. melanogaster) | y,w,hs-FLP;LexAop-RFPnls; UAS-GFPnls | Buttitta lab stocks | n/a | hs-FLP used with with CoinFLP nuclear GFP and RFP |
| Genetic reagent (D. melanogaster) | ey-FLP | Bloomington Drosophila Stock Center | BDSC 5576 | ey-FLP |
| Genetic reagent (D. melanogaster) | y,sev,w;UAS-cdc6RNAi | Bloomington Drosophila Stock Center | BDSC 55734 | cdc6KD |
| Genetic reagent (D. melanogaster) | w; UAS-gemininRNAi | Bloomington Drosophila Stock Center | BDSC 30929 and 50720 | gemininKD |
| Genetic reagent (D. melanogaster) | w1118;GUS-p53 | Bloomington Drosophila Stock Center | BDSC 6584 | UAS-p53WT |
| Genetic reagent (D. melanogaster) | y,w1118; UAS-p53 259N | Bloomington Drosophila Stock Center | BDSC 6582 | UAS-p53DN |
| Antibody | anti-ELAV (rat monoclonal) |
Developmental Studies Hybridoma Bank | Rat-ELAV-7E8A10 | 1: 100 |
| Antibody | anti-pH2AV (mouse monoclonal) |
Developmental Studies Hybridoma Bank | UNC93-5.2.1 | 1: 100 |
| Antibody | anti-Repo (mouse monoclonal) |
Developmental Studies Hybridoma Bank | 8D12 | 1: 100 |
| Antibody | anti-Lamin (mouse monoclonal) |
Developmental Studies Hybridoma Bank | ADL67.10 | 1: 100 |
| Antibody | Alexa Fluor 568 anti-mouse (goat polyclonal) |
ThermoFisher | A11031 | 1: 1000 |
| Antibody | Alexa Fluor 568 anti-rat (goat polyclonal) | ThermoFisher | A11077 | 1: 1000 |
| Antibody | Alexa Fluor 488 anti-mouse (goat polyclonal) |
ThermoFisher | A11029 | 1: 1000 |
| Antibody | Alexa Fluor 488 anti-rat (donkey polyclonal) |
ThermoFisher | A21208 | 1: 1000 |
| Other | DAPI | Sigma-Aldrich | D9542 | 1: 1000 |
| Other | Dye-cycle violet | ThermoFisher | V35003 | 2: 1000 |
| Other | Sytox Green | ThermoFisher | S7020 | 2: 1000 |
| Other | Propidium Iodide | Sigma-Aldrich | P4170 | 2.25: 1000 |