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Effect of Inhibiting the Pannexin 1 Channel on Hemi-channel Function. Representative images depicting PI dye uptake (red) by untreated granulosa cells (b) and granulosa cells treated with 10Panx (e). Cells were nuclear stained with Hoechst33342 (blue, a & d) and overlayed (c & f). Bars = 400 μm. g The relative number of PI positive cells in control or 10Panx treated granulosa cells. *Denotes a significant difference, p < 0.05. Error bars are ± SD from mean
