Figure 5.

Effect of HBNs 1–9 on caspase-3 activity in SH-SY5Y neuroblastoma cells after IR. Bars show caspase 3 activity expressed as ΔAFU/μg protein/min after OGD (4 h) and IR (24 h) alone or treated with HBNs 1–9, PBN and NAC, at the indicated concentrations. Values are the mean ± SEM of three experiments, each one performed in triplicate, and compare the effect of OGD and IR on respective controls (C4h and C24h, respectively) (red ***) or the effect of the different compounds after IR treatment with IR alone, in the absence of these compounds (black ***). Data were statistically analyzed by one-way ANOVA, followed by Holm-Sidak as test post hoc. *P < 0.05, **P < 0.01 and ***P < 0.001. UAF = Arbitrary Fluorescent Units.