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. 2020 Aug 25;10:14180. doi: 10.1038/s41598-020-71027-5

Figure 6.

Figure 6

LPS decreases the cytoplasmic fraction of of NFκB in the hippocampus. (a,b) Western blotting densitometric analysis (arbitrary units) and representative Western blots for p65 (NFκB) and β-actin in the hypothalamus (a) (blue bars) (n = 5 mice/group) and hippocampus (b) (red bars) of the α2+/+ (n = 5) and α2+/G301R (n = 5) animals 4 h after LPS treatment. All data are presented as the mean ± SEM. *p < 0.05 (Kruskal–Wallis test followed by Dunn’s post hoc test). RT-qPCR analysis of (c,d) Il-6 and (e,f) Il-1β relative to β-actin expression in astrocytes isolated from the hypothalamus (c,e) and hippocampus (d,f) showed significant increases after LPS treatment for all except Il-1β in hippocampus of α2+/G301R mice (n = 3 for both groups). All data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 (Kruskal–Wallis test followed by Dunn’s post hoc test).