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. 2020 Aug 25;11:4078. doi: 10.1038/s41467-020-17930-x

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Experimental design. Mice (8~12 months old males) underwent tMCAO for 90 min. The occlusion and reperfusion were confirmed by a laser speckle imager (LSI). Recipient mice were then randomized into two groups: a control group receiving sham surgery (white circles, n = 5) and a blood replacement (BR) group receiving 500 µl of blood from healthy donors (black circles, n = 5). Blood was replaced into stroke mice at 6.5~7 h post stroke. The same volume of blood was withdrawn from the recipient stroke mice during blood transfusion. Brains were perfused and analyzed at 8 and 23 h after ischemia induction. Blood from naive donors reduced numbers of total inflammatory cells in ischemic hemispheres at b 8 and c 23 h post stroke. Representative flow cytometry images for neutrophils gated on propidium iodide (PI)⁻CD45⁺CD11b⁺Gr-1⁺ populations and macrophages gated on PI⁻CD45⁺CD11b⁺Gr-1⁻ populations in hemispheres at d 8 and e 23 h post stroke. Blood from naive donors reduced numbers of absolute neutrophil counts in ischemic hemispheres at f 8 and g 23 h post stroke. Blood from naive donors reduced numbers of absolute macrophage counts in ischemic hemispheres at h 8 and i 23 h post stroke. Data were presented as means ± SEM. One-way ANOVA followed by post hoc Tukey’s tests. Source data are provided as a Source Data file.