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. 2020 Aug 25;10(9):403. doi: 10.1007/s13205-020-02386-z

Table 1.

qPCR primers

Gene name Sequence (5′ to 3′) Fragments/bp Nnnealing temperature/℃
SPP1 S: 5′ CAAAGCTGCCAGGAAGCTCAT 3' 171 60
A: 5′ TCCACCTCAGGGCTGTCAAA 3'
CAV3 S:5′ CTTCCTCTTCGCCCTCATCT 3' 182 60
A:5′ CCTTCCGCAGCATAACCCT 3'
PTK2 S: 5′ GCAGTCTGAGGAGGTTCACT 3' 123 60
A: 5′ TTGGGCAGGTACCGAATTCT 3'
PDGFB S: 5′ TGGACAGCACCAATGCCAA 3' 192 60
A: 5′ GGTCCTCCAAAGGCACGAT 3'
PDGFRα S: 5′ CTTGGCAGCTCGTAATGTCC 3' 125 60
A: 5′ TTACTGGGAGGAAGGTGCTG 3'
RAC2 S: 5′ TGCTGGACAAGAGGATTACGA 3' 165 60
A: 5′ ACAAGGATGATGGGAGTGCTG 3'
MYOD S: 5′GCTACTACACGGAATCACCAAAT 3' 21 60
A: 5′ CTGGGCTCCACTGTCACTCA 3'
MYOG S: 5′ CGGAGGCTGAAGAAGGTGAA 3' 314 60
A: 5′CGGTCCTCTGCCTGGTCAT 3'
RASGRF1 S: 5′ TTTGACATCCTGCTGCCCAT 3' 110 60
A: 5′ AGCTTGTCGAAGTCACGGTT 3'
ß-actin S: 5 'GAGAAATTGTGCCTGACATCA 3' 152 60
A: 5 'CCTGAACCTCTCATTGCCA 3'