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. 2020 Aug 25;10:14189. doi: 10.1038/s41598-020-71167-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Knock down of AQP4 and cell atrophy. (a) AQP4 protein expression in AQP4 shRNA-transfected C2C12 cells. Differentiated C2C12 cells were transfected with a different dose of shAQP4 for 48 h, and the cell lysates were used for western blot analysis for AQP4 protein expression. (b) Morphology of AQP4 shRNA-transfected C2C12 cells during differentiation. C2C12 cells were differentiated for 7 days with or without AQP4 shRNA. (c) Flow cytometry analysis in AQP4 shRNA-transfected C2C12 cells. Differentiated C2C12 cells were transfected with shAQP4 for 48 h, and the size and granularity (complexity) of cells were determined by forward and side scatter (FSC and SSC) gating, respectively. Forward scatter (FSC) represent the cell size (d), and side scatter (SSC) data represent the complexity or granularity of the cell (e).