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. 2020 Aug 25;10:14178. doi: 10.1038/s41598-020-71137-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The construction of pET21a-SpG3 and the expression of SpG3. (A) The structure of redesigned gene of SpG3. (B) The detection of pET21a-SpG3 by enzyme digestion. Line 1 was marker; pET21a-SpG3 in line 2 was digested with restriction enzyme EcoR I and Xho I. (C) The expression of SpG3 induced by 0.5 mM IPTG for 12 h and shaking at 30 °C. Line 1, marker; Line 2, control sample; Line 3, the sample with stimulation for 12 h. (D) Western blot. The membrane was incubated with monoclonal antibodies (mAb) against His-tag (1:3,000). Line 1, control sample; Line 2, the purified sample with stimulation for 12 h.