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. 2020 Aug 25;10:14178. doi: 10.1038/s41598-020-71137-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The sensitivity and specificity for detection of SLE by AOL-conjugated colloidal gold Dot-ELISA assay. (A) Optimal ratio of colloidal gold and AOL. To determine the optimal ratio of colloidal gold and AOL for conjugation, a fixed amount of colloidal gold (100 μL) and 10% NaCl (10 μL) was mixed with an increasing amount from 0 to 80 μg/mL, of AOL. As the concentration of the antibodies increased, the color of the AOL-conjugated colloid gold became red. (B) The optimization of the minimum amount of AOL for stabilizing colloidal gold solution. (C) The specificity of AOL-conjugated colloidal gold Dot-ELISA. The sera (dilution from 1: 50 to 1:1,000) of Fut8^+/+ mice and Fut8^−/− mice were analyzed by AOL-conjugated colloidal gold Dot-ELISA. (D) The optimization of AOL-conjugated colloidal gold Dot-ELISA. SLE sera with different ANA titers (from 1:100 to 1:1,000) were analyzed by AOL-conjugated colloidal gold Dot-ELISA. (E) Detection of SLE sera by AOL conjugated colloidal gold Dot-ELISA.