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. 2020 Aug 25;10:14156. doi: 10.1038/s41598-020-70824-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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E2F-responsive cell cycle genes are downregulated in Zfp148-deficient cells. (A) Heat-map showing expression profiles of the top 300 genes that were downregulated in the absence of Zfp148 (blue colour indicates downregulation, red indicates upregulation). Bar showing the rank order of 59 validated E2F target genes according to the heat-map. (B) Table showing enriched GO terms on Trp53^+/+ (left) or Trp53^−/− (right) genetic background in rank order. (C) Real-time RT-PCR analysis of 17 genes selected from the top 300-set in wild-typeand Zfp148^gt/gt MEFs on Trp53^+/+ (n = 3) or Trp53^+/− (n = 4) genetic background. Red box indicates genes linked to the GO-term “Cell Cycle”. * and # indicate significant differential expression in wild-type versus Zfp148^gt/gt and Zfp148^gt/gt versus Zfp148^gt/gtTrp53^+/− MEFs, respectively. (D) Graphs showing log2 expression ratios of the top-300 downregulated genes in lungs (left), brains (middle), and bone marrow-derived macrophages (right) from newborn Zfp148^gt/gt mice compared to controls (n = 3). Red colour indicates genes linked to the GO term “Cell Cycle”.