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. 2020 Aug 25;10:14161. doi: 10.1038/s41598-020-70977-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transient over-expression of MYBC1 and WRKY44 in Nicotiana tabacum leaves for anthocyanin accumulation. (A) Digital image of N. tabacum leaves 7 days after transient over-expression of MYBC1 and WRKY44 from Actinidia melanandra, A. purpurea and MaMe Red with MYB110 from A. purpurea as a positive control. (B) HPLC measurement of anthocyanin from transient over-expression of MYBC1 and WRKY44 from (A). Data were shown as means ± SEM of three individual experiments.