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. 2020 Aug 25;10:14161. doi: 10.1038/s41598-020-70977-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Dual luciferase promoter activation assays testing the ability of MYBC1 and WRKY44 for promoter activation. (A) F3′H promoters isolated from Actinidia melanandra, A. purpurea, MaMe Red and MaMe Yellow were tested for activation by the MYBC1 and WRKY44 cloned from the respective species. (B) F3′5′H promoters isolated from A. melanandra, A. purpurea, MaMe Red and MaMe Yellow were tested for activation by the MYBC1 and WRKY44 cloned from the respective species. Empty vector and A. purpurea MYB110 were included as control. Data were shown as means ± SEM of four biological replicates. Statistical significance against the empty vector control: p < 0.05*, p < 0.01**, and p < 0.001***.