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. 2020 Aug 25;10:14161. doi: 10.1038/s41598-020-70977-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Stable over-expression of GUS, MYB110, MYBC1, and WRKY44 in Actinidia arguta. (A) Callus formation of GUS, MYB110, MYBC1, WRKY and the co-infiltration of MYBC1/WRKY44 eight weeks after Agrobacteria-mediated over-expression in A. arguta leaf explant. (B) Proanthocyanin and anthocyanin analysis of the calli sampled from (A). (C) Gene expression analysis of the transgenes MYB110, MYBC1, WRKY44 and genes involved in the proanthocyanin and anthocyanin pathway in the callus from stably transformed calli. Data were shown as means ± SEM of three biological replicates. Statistical significance against the GUS control: p < 0.05*, p < 0.01**, and p < 0.001***.