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. 2020 Aug 25;6:78. doi: 10.1038/s41420-020-00313-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Mitochondria were analyzed by confocal microscopy with anti-PDH antibodies (a mitochondrial matrix protein, in green) and DAPI. White arrows indicate swollen mitochondria. b Histogram of mitochondrial aspect ratios. c Average mitochondrial size (n = 4334 mitochondria from the control group, n = 709 mitochondria from with Fen group). d Average mitochondrial aspect ratio (n = 4334 mitochondria from the control group, n = 709 mitochondria from the Fen group). e Quantitative analysis of PDH fluorescence. f The represent image of TEM. Black arrows indicate swollen mitochondria. g In vitro and h in vivo protein levels of Drp1, Mfn-1, and Mfn-2 detected by Western blotting (above panel) and quantitative analysis of protein-band intensities (below panel). Figure 2f and h are represent same samples, therefore, Actin in Fig. 2f and h are completely identical. For all quantitative/statistical analysis, *p < 0.05; **p < 0.01; ***p < 0.001, and “ns” denotes no significant difference. All data are presented as means ± SEMs. Scale bars are 20 μm in a and 500 nm in f.