Figure 8.

(a) Cell viability study of MDA-MB-231 cells showing the effect of the treatment time when incubated with drug-loaded and unloaded PLGA-PEG microspheres after for a period of 240 h with MDA-MB-231 cells acting as a control. (b) Representative confocal images of MDA-MB-231 cells after 5 h incubation with respective drug-loaded PLGA-PEG microspheres at 37 °C. Red staining reveals actin-filaments and green staining indicates vinculin. All cells were stained and imaged under the same conditions. White arrows indicate the initiation of cytoskeleton disruption/disintegration (n = 3, ^$p < 0.05 vs. control).