Figure 1.

ASCC Is Required for Aborting Translation at a Ribosome Stall

(A) The dual-fluorescence reporter used to study translational stalling.

(B) Cells were treated with control (gray shaded) or ASCC3-targeting (red traces) siRNAs for 72 h before induction of the stably integrated reporter with doxycycline for 20 h. The RFP:GFP ratio as determined by flow cytometry is plotted as a histogram.

(C) Immunoblots of whole cell lysates after treatment with the indicated siRNAs for 72 h. A non-specific product detected by the ASCC1 antibody is indicated with an asterisk in this and subsequent figures.

(D) Stalling assay as in (B) after treatment with the indicated siRNAs for 72 h.

See also Figures S1 and S5.