Figure 1.

TCP-Seq in Human Cells: Full Ribosome FPs

(A) Outline of translation complex profile sequencing (TCP-seq) and selective (Sel)-TCP-seq. 40S, small ribosomal subunit; 80S, ribosome; FOI, factor of interest.

(B) Human 80S footprint (FP) length versus 5′ end position relative to the first nucleotide (position 0) of start (left, 10,778 sites) or stop codons (right, 16,803 sites). The color scale represents FP count as indicated on the right.

(C) Length distribution of human 80S FPs within 9,241 coding sequences (CDS; excluding start and stop codon-associated FPs).

(D) Schematic to facilitate interpretation of (B). Bars illustrate FP lengths as opposed to 5′ end position only.

Shown are representative data from biological replicate 3 (see Table S1 for a full listing).