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. 2020 Aug 20;79(4):546–560.e7. doi: 10.1016/j.molcel.2020.06.004

Figure 4.

Figure 4

Sel-TCP-Seq Resolves Individual Start Codon Recognition Steps

(A) Yeast start codon-aligned, unselected, and FOI::40S FP 3′ end position frequencies (5,994 sites; shading around lines indicates ± standard deviation). Gray vertical bars are shown as in Figure 2D.

(B) Suggested influence of queuing 40Ses on co-immunoprecipitation (coIP) efficiency.

(C) 5′ end position frequencies of unselected 40S FPs ending at specific 3′ end positions, as indicated to the right of each row. Selection matches the gray bars in (A) but respects partition of the +15 to +18 area into two peaks. For orientation, bars representing the −30 and −13 positions are highlighted in black. Error bars indicate ± standard deviation.

(D) As (C) but for eIF2β::40Ss.

(E) As (C) but for eIF3a::40Ss.

Shown are averages of yeast replicates 1 and 2.