Figure 5.

Sel-TCP-Seq Dissects REI-Mediated Control of GCN4 mRNA Translation

(A) Yeast unselected 40S and 80S FP coverage frequency along the 5′ UTR of GCN4 mRNA (schematic with regulatory elements shown on top). uORF locations are emphasized by vertical gray shading; positions of REI-promoting elements (RPEs) i, ii, and iv are further indicated by colored bars on the x axis. Averages of replicates 1-1, 1-2, and 1-3 are shown.

(B) Schematic of the yeast GCN4 translational control mechanism.

(C) As (A) but showing eIF3a::40S FP coverage frequency (the unselected 40S track is indicated as a dashed line). Averages of replicates 1 and 2 are shown.

(D) As (C), but eIF2β::40S and unselected 40S tracks are shown.

Error bars in (A), (C), and (D) indicate ± standard deviation.