Table 2.
Ames test results
| Type of test | Experimental test system | Test substance | Exposure conditions | Result | WG considerations and conclusion | Reference |
|---|---|---|---|---|---|---|
| Ames test | S. typhimurium TA98, TA100, TA1535, TA1537, E. coli WP2 uvrA |
triazine amine IN‐A4098‐005 (4‐Methoxy‐6‐methyl‐1,3,5‐triazin‐2‐amine) (98.7%) Vehicle: DMSO |
Plate incorporation assay +/− rat liver S9 First exp. 1.5, 5, 15, 50, 150, 500, 1,500, 5,000 μg/plate; Confirmatory exp.: 50, 150, 500, 1,500, 5,000 μg/mL |
No treatment related increase in revertant colonies; no visible precipitation of the test substance, and no evidence of toxicity to bacteria |
GLP compliant According to OECD TG 471 (1997) Negative |
Wagner and Van Dyke (2009) |
| Ames test | S. typhimurium TA98, TA100, TA1535, TA1537, E. coli WP2 uvrA |
triazine amine Aminotriazine, batch 040101 (99%) Vehicle: suspended in DMSO |
Plate incorporation and pre‐incubation assay Six doses tested, from 31.6 to 5,000 μg/plate (+/− rat liver S9) |
No treatment related increase in revertant colonies; no visible precipitation of test substance, no evidence of toxicity to bacteria |
GLP compliant According to OECD TG 471 (1997) Negative |
Donath (2011) |
| Ames test | S. typhimurium TA98, TA100, TA1535, TA1537, TA102 |
triazine amine IN‐A4098 (2‐amino‐4‐methoxy‐6‐methyl‐1,3,5‐triazine) (99.5%) Vehicle: dissolved in DMSO (at 10 mg/mL) |
Six doses tested (+/− rat liver S9) Plate incorporation from 31.6 to 5,000 μg/plate Pre‐incubation assay from 10 to 3,160 μg/plate |
No increased revertant colonies at any dose; signs of toxicity to bacteria (reduced background lawn) at the top dose |
GLP compliant According to OECD TG 471 (1997) Negative |
Flügge (2011a) |
| Ames test |
S. typhimurium TA98, TA100, TA1535, TA1537 E. coli WP2uvrA |
triazine amine CGA 150829 tech (99.4%) Vehicle: suspended in DMSO |
Plate incorporation assay Five doses tested, from 312.5 to 5,000 μg/plate +/− rat liver S9 in two separate assays |
No treatment related increase in revertant colonies; no evidence of toxicity to bacteria |
GLP compliant According to OECD TG 471 (1983) Negative |
Geleick (1991) |
| Ames test |
S. typhimurium TA98, TA100, TA1535, TA1537 E. coli WP2uvrA |
triazine amine IN‐A4098 (2‐amino‐4‐methoxy‐6‐methyl‐1,3,5‐triazine) (97%) Vehicle: suspended in water + 0.15% agar |
Plate incorporation and pre‐incubation assay Five doses tested, from 50 to 5,000 μg/plate +/− rat liver S9 |
No treatment related increase in revertant colonies; no evidence of toxicity to bacteria |
GLP compliant According to OECD TG 471 (1997) Negative |
May (2011) |
| Ames test |
S. typhimurium TA98, TA100, TA1535, TA1537 E. coli WP2uvrA |
triazine amine AE F059411 tech (2‐amino‐4‐methoxy‐6‐methyl‐1,3,5‐triazine) (99.6%) Vehicle: suspended in DMSO |
Plate incorporation and pre‐incubation assay Five doses tested, from 50 to 5,000 μg/plate +/− rat liver S9 |
No treatment related increase in revertant colonies; no evidence of toxicity to bacteria |
GLP compliant According to OECD TG 471 (1997) Negative |
Stammberger and Braun (1988) |
DMSO: dimethyl sulfoxide; S9: metabolic activation.