Table 3.

List of toxicological studies with the NF

Test item	Reference	Type of study	Test system	Dose
NF	National Medicines Institute (2016)	Bacterial reverse mutation test	Salmonella Typhimurium TA97, TA98, TA100 and TA102	Up to 5,000 μg Se/plate (absence and presence of S9 mix)
NF	Rahden‐Staroń et al. (2010)	Bacterial reverse mutation test	S. Typhimurium TA97a, TA98, TA100, TA1535 and TA102	Up to 5% corresponding to 5,000 μg Se/plate (absence and presence of S9 mix)
NF micelles	National Medicines Institute (2018)	In vitro mammalian cell micronucleus test	Mouse fibroblasts NCTC clone 929, ATCC	2 mg/mL (absence and presence of S9 mix)
Selol (unknown % selenium)	Jastrzębski et al. (1995)	Acute toxicity study	Male Wistar rats	Up to 200 mg/kg bw oral gavage Up to 100 mg/kg bw intraperitoneal Up to 500 mg/kg bw subcutaneous
		Cumulative toxicity study	Male Wistar rats	Up to 154 mg/kg bw oral gavage
NF	Institute of Industrial Organic Chemistry (2017)	90‐day repeated dose oral toxicity study	Wistar rats	Up to 32 mg/kg bw per day

bw: body weight; GLP: good laboratory practice; OECD TG: Organisation for Economic Co‐operation and Development – Test guideline.